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Rabbit anti-SAP49 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to SAP49

CAS Number

9007-83-4

Synonyms

SAP49; Splicing factor 3B subunit 4; Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49; SAP 49

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SAP49. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of SAP49 expression in HEK293T (A), Jurkat (B) whole cell lysates.

IHC description

Immunohistochemical analysis of SAP49 staining in human lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

10262

Gene ID (Mouse)

107701

Gene ID (Rat)

295270

SwissProt (Human)

Q15427

SwissProt (Mouse)

Q8QZY9

SwissProt (Rat)

Q6AYL5

Available Sizes

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