Rabbit anti-NF-kappaB p100 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to NF-kappaB p100
CAS Number
9007-83-4
Synonyms
LYT10; Nuclear factor NF-kappa-B p100 subunit; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10 protein; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt-10; Lyt10
Host
Rabbit
Reactivity
H, M, R, P
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p100. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of NF-kappaB p100 expression in Jurkat (A), MCF7 (B), HeLa (C) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of NF-kappaB p100 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
4791
Gene ID (Mouse)
18034
SwissProt (Human)
Q00653
SwissProt (Mouse)
Q9WTK5
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC040391-500 1x 500 µL
CD81 / TAPA-1 (1.3.3.22), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040916-500 1x 500 µL
Mucin 6 (MUC6) (MUC6/916), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC050485-500 1x 500 µL
ODC-1 (Ornithine Decarboxylase-1) (ODC1/485), CF405M conjugate, 0.1mg/mL
Sign In for Pricing
View Details 