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Rabbit anti-RNF125 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to RNF125

CAS Number

9007-83-4

Synonyms

E3 ubiquitin-protein ligase RNF125; RING finger protein 125; T-cell RING activation protein 1; TRAC-1

Host

Rabbit

Reactivity

H, M, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RNF125. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of RNF125 expression in HEK293T (A), THP1 (B), 129Sv (C) whole cell lysates.

IHC description

Immunohistochemical analysis of RNF125 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

54941

Gene ID (Mouse)

67664

SwissProt (Human)

Q96EQ8

SwissProt (Mouse)

Q9D9R0

Available Sizes

Frequently Asked Questions

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