Rabbit anti-RNF125 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to RNF125
CAS Number
9007-83-4
Synonyms
E3 ubiquitin-protein ligase RNF125; RING finger protein 125; T-cell RING activation protein 1; TRAC-1
Host
Rabbit
Reactivity
H, M, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human RNF125. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of RNF125 expression in HEK293T (A), THP1 (B), 129Sv (C) whole cell lysates.
IHC description
Immunohistochemical analysis of RNF125 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
54941
Gene ID (Mouse)
67664
SwissProt (Human)
Q96EQ8
SwissProt (Mouse)
Q9D9R0
Available Sizes
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