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Rabbit anti-E2F1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to E2F1

CAS Number

9007-83-4

Synonyms

RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1

Host

Rabbit

Reactivity

H, M, R, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human E2F1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of E2F1 expression in MCF7 (A), A549 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of E2F1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1869

Gene ID (Mouse)

13555

SwissProt (Human)

Q01094

SwissProt (Mouse)

Q61501

SwissProt (Rat)

O09139

Available Sizes

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