Rabbit anti-E2F1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to E2F1
CAS Number
9007-83-4
Synonyms
RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
Host
Rabbit
Reactivity
H, M, R, Mk
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human E2F1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of E2F1 expression in MCF7 (A), A549 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of E2F1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1869
Gene ID (Mouse)
13555
SwissProt (Human)
Q01094
SwissProt (Mouse)
Q61501
SwissProt (Rat)
O09139
Available Sizes
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