Rabbit anti-Calponin 2 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Calponin 2
CAS Number
9007-83-4
Synonyms
Calponin-2; Calponin H2, smooth muscle; Neutral calponin
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Calponin 2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Calponin 2 expression in COLO205 (A) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of Calponin 2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
1265
Gene ID (Mouse)
12798
SwissProt (Human)
Q99439
SwissProt (Mouse)
Q08093
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC470012-500 1x 500 µL
Tyrosinase (T311), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC040630-500 1x 500 µL
Mucin 1 / EMA / Episialin / CD227 (VU-2G7), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNUB0026-500 1x 500 µL
ACTH (Adrenocorticotrophic Hormone) (AH26), 0.2mg/mL
Sign In for Pricing
View Details 
BNC041388-100 1x 100 µL
Ferritin, Light Chain (FTL) (Microglia Marker) (FTL/1388), CF405S conjugate, 0.1mg/mL
Sign In for Pricing
View Details