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Rabbit anti-ATG4B Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ATG4B

CAS Number

9007-83-4

Synonyms

APG4B; AUTL1; KIAA0943; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; hAPG4B

Host

Rabbit

Reactivity

H, M, Mk

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ATG4B. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ATG4B expression in Jurkat (A) whole cell lysates.

IHC description

Immunohistochemical analysis of ATG4B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

23192

Gene ID (Mouse)

66615

SwissProt (Human)

Q9Y4P1

SwissProt (Mouse)

Q8BGE6

Available Sizes

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