Rabbit anti-PI3K p85 alpha Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PI3K p85 alpha
CAS Number
9007-83-4
Synonyms
GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of PI3K p85 alpha expression in HeLa (A) whole cell lysates.
IHC description
Immunohistochemical analysis of PI3K p85 alpha staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5295
Gene ID (Mouse)
18708
Gene ID (Rat)
25513
SwissProt (Human)
P27986
SwissProt (Mouse)
P26450
SwissProt (Rat)
Q63787
Available Sizes
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