Rabbit anti-GSK3 alpha/beta (pY279/216) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GSK3 alpha/beta (pY279/216)
CAS Number
9007-83-4
Synonyms
GSK3A; Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Host
Rabbit
Reactivity
H, M, R, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GSK3 alpha/beta (pY279/216) . The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of GSK3 alpha/beta (pY279/216) expression in A549 insulin-treated (A), NIH3T3 insulin-treated (B) whole cell lysates.
IHC description
Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
2931
Gene ID (Mouse)
606496; 56637
Gene ID (Rat)
50686; 84027
SwissProt (Human)
P49840; P49841
SwissProt (Mouse)
Q2NL51; Q9WV60
SwissProt (Rat)
P18265; P18266
Available Sizes
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