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Rabbit anti-CRK Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CRK

CAS Number

9007-83-4

Synonyms

Adapter molecule crk; Proto-oncogene c-Crk; p38

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CRK. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CRK expression in HeLa (A), K562 (B), NIH3T3 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of CRK staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1398

Gene ID (Mouse)

12928

Gene ID (Rat)

54245

SwissProt (Human)

P46108

SwissProt (Mouse)

Q64010

SwissProt (Rat)

Q63768

Available Sizes

Frequently Asked Questions

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