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Rabbit anti-CDK8 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CDK8

CAS Number

9007-83-4

Synonyms

Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35

Host

Rabbit

Reactivity

H, M, R, Z

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK8. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CDK8 expression in HepG2 (A), A549 (B), Raw264.7 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1024

Gene ID (Mouse)

264064

SwissProt (Human)

P49336

SwissProt (Mouse)

Q8R3L8

Available Sizes

Frequently Asked Questions

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