Rabbit anti-CDK8 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CDK8
CAS Number
9007-83-4
Synonyms
Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35
Host
Rabbit
Reactivity
H, M, R, Z
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDK8. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CDK8 expression in HepG2 (A), A549 (B), Raw264.7 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of CDK8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1024
Gene ID (Mouse)
264064
SwissProt (Human)
P49336
SwissProt (Mouse)
Q8R3L8
Available Sizes
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