Rabbit anti-p130 Cas (pY249) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to p130 Cas (pY249)
CAS Number
9007-83-4
Synonyms
CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
Host
Rabbit
Reactivity
H, M, R, B
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human p130 Cas. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of p130 Cas (pY249) expression in HEK293T EGF-treated (A), NIH3T3 EGF-treated (B), Raw264.7 EGF-treated (C), PC12 EGF-treated (D) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of p130 Cas (pY249) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .
Gene ID (Human)
9564
Gene ID (Mouse)
12927
Gene ID (Rat)
25414
SwissProt (Human)
P56945
SwissProt (Mouse)
Q61140
SwissProt (Rat)
Q63767
Available Sizes
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