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Rabbit anti-BAG4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to BAG4

CAS Number

9007-83-4

Synonyms

SODD; BAG family molecular chaperone regulator 4; BAG-4; Bcl-2-associated athanogene 4; Silencer of death domains

Host

Rabbit

Reactivity

H, M, R, B

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BAG4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of BAG4 expression in HeLa (A), MOLT4 (B), THP1 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of BAG4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

9530

Gene ID (Mouse)

67384

SwissProt (Human)

O95429

SwissProt (Mouse)

Q8CI61

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