Rabbit anti-MCL1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MCL1

CAS Number

9007-83-4

Synonyms

BCL2L3; Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT

Host

Rabbit

Reactivity

H, D, P

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MCL1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of MCL1 expression in Ramos (A), JAR (B), K562 (C), Jurkat (D), Raji (E), HeLa (F) whole cell lysates.

IHC description

Immunohistochemical analysis of MCL1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.