T4 Polynucleotide kinase (10 U/μL)

T4 Polynucleotide Kinase (T4 PNK) is a polynucleotide 5'-hydroxyl kinase that catalyzes the transfer of the γ-phosphate from ATP to the 5'-OH group of oligonucleotide chains (double- or single-stranded DNAs or RNAs) and nucleoside 3'-monophosphates, and the reaction is reversible. Has 3'- phosphatase activity, hydrolyzes the 3'-phosphate group from the 3'-phosphate end of oligonucleotides, deoxy3'-monophosphate nucleosides and deoxy3'-diphosphate nucleosides. In the presence of ADP, T4 PNK has 5'- phosphatase activity, catalyzing the exchange of 5'-P-oligo/polynucleotide and ATP terminal 5'-phosphate groups. It is suitable for DNA library construction and probes end labeling.