Rabbit anti-BRCA1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to BRCA1
CAS Number
9007-83-4
Synonyms
RNF53; Breast cancer type 1 susceptibility protein; RING finger protein 53
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human BRCA1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BRCA1 expression in HeLa (A), mouse kidney (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of BRCA1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
672
Gene ID (Mouse)
12189
Gene ID (Rat)
497672
SwissProt (Human)
P38398
SwissProt (Mouse)
P48754
SwissProt (Rat)
O54952
Available Sizes
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