Rabbit anti-BCL2 (pT69) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to BCL2 (pT69)
CAS Number
9007-83-4
Synonyms
Apoptosis regulator Bcl-2
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human BCL2. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of BCL2 (pT69) expression in HepG2 UV-treated (A), HeLa (B), HL60 (C), THP1 (D), Jurkat paclitaxel-treated (E), NIH3T3 H2O2-treated (F), PC12 H2O2-treated (G) whole cell lysates.
IHC description
Immunohistochemical analysis of BCL2 (pT69) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
596
Gene ID (Mouse)
12043
Gene ID (Rat)
24224
SwissProt (Human)
P10415
SwissProt (Mouse)
P10417
SwissProt (Rat)
P49950
Available Sizes
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