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Rabbit anti-ZNF346 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ZNF346

CAS Number

9007-83-4

Synonyms

JAZ; Zinc finger protein 346; Just another zinc finger protein

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human ZNF346

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ZNF346 expression in A549 (A), MCF7 (B), mouse brain (C) whole cell lysates.

IHC description

Immunohistochemical analysis of ZNF346 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of ZNF346 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

23567

Gene ID (Mouse)

26919

SwissProt (Human)

Q9UL40

SwissProt (Mouse)

Q9R0B7

Available Sizes

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