Rabbit anti-Nucleophosmin Antibody
Product Specifications
Background
Rabbit polyclonal antibody to Nucleophosmin
CAS Number
9007-83-4
Synonyms
NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
Host
Rabbit
Reactivity
H, Rb
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Nucleophosmin. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC, IP
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of Nucleophosmin expression in A549 (A), LNCaP (B), HeLa (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Nucleophosmin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4869
SwissProt (Human)
P06748
Available Sizes
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