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Rabbit anti-CYB5R3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CYB5R3

CAS Number

9007-83-4

Synonyms

DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; Diaphorase-1

Host

Rabbit

Reactivity

H, D

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CYB5R3. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IP

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CYB5R3 expression in HeLa (A) whole cell lysates.

IHC description

Immunohistochemical analysis of CYB5R3 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

1727

SwissProt (Human)

P00387

Available Sizes

Frequently Asked Questions

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