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Mouse anti-GRP78 Antibody

Product Specifications

Background

Mouse monoclonal antibody to GRP78

CAS Number

9007-83-4

Synonyms

GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca (2+) -binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP

Host

Mouse

Reactivity

H, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human GRP78. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IHC

Concentration

1mg/mL

Dilution

WB (1/1000 - 1/2000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GRP78 expression in Hela (A), rat liver (B) whole cell lysates.

IHC description

Immunohistochemical analysis of GRP78 staining in human colon cancer, human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

3309

SwissProt (Human)

P11021

Available Sizes

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