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Rabbit anti-Herc3 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Herc3

CAS Number

9007-83-4

Synonyms

KIAA0032; Probable E3 ubiquitin-protein ligase HERC3; HECT domain and RCC1-like domain-containing protein 3

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human Herc3

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Herc3 expression in A431 (A), mouse lung (B) whole cell lysates.

IHC description

Immunohistochemical analysis of Herc3 staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

8916

SwissProt (Human)

Q15034

Available Sizes

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