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Rabbit anti-EPC1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to EPC1

CAS Number

9007-83-4

Synonyms

Enhancer of polycomb homolog 1

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human EPC1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of EPC1 expression in MCF7 (A), mouse lung (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of EPC1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

80314

Gene ID (Mouse)

13831

SwissProt (Human)

Q9H2F5

SwissProt (Mouse)

Q8C9X6

Available Sizes

Frequently Asked Questions

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