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Rabbit anti-ING5 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to ING5

CAS Number

9007-83-4

Synonyms

Inhibitor of growth protein 5; p28ING5

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human ING5

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of ING5 expression in Jurkat (A), A549 (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of ING5 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

84289

Gene ID (Mouse)

66262

SwissProt (Human)

Q8WYH8

SwissProt (Mouse)

Q9D8Y8

Available Sizes

Frequently Asked Questions

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