What is the catalog number for Rabbit anti-GRASP65 Antibody?

The catalog number for Rabbit anti-GRASP65 Antibody is DL96847A.

What sizes are available for Rabbit anti-GRASP65 Antibody?

Rabbit anti-GRASP65 Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-GRASP65 Antibody come in?

Rabbit anti-GRASP65 Antibody is available in 2 different pack sizes.

Is Rabbit anti-GRASP65 Antibody in stock?

Rabbit anti-GRASP65 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-GRASP65 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GRASP65

CAS Number

9007-83-4

Synonyms

GOLPH5; GRASP65; Golgi reassembly-stacking protein 1; Golgi peripheral membrane protein p65; Golgi phosphoprotein 5; GOLPH5; Golgi reassembly-stacking protein of 65 kDa; GRASP65

Host

Rabbit

Reactivity

Immunogen

Recombinant full length protein of human GRASP65

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/10 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of GRASP65 expression in A549 (A), U937 (B), mouse kidney (C), mouse heart (D), rat liver (E) whole cell lysates.

IHC description

Immunohistochemical analysis of GRASP65 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of GRASP65 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.