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Rabbit anti-CAT1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to CAT1

CAS Number

9007-83-4

Synonyms

CAT1; Carnitine O-acetyltransferase; Carnitine acetylase; Carnitine acetyltransferase; CAT; CrAT

Host

Rabbit

Reactivity

H, M

Immunogen

Recombinant full length protein of human CAT1

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of CAT1 expression in mouse skeletal muscle (A) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of CAT1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

1384

Gene ID (Mouse)

12908

SwissProt (Human)

P43155

SwissProt (Mouse)

P47934

Available Sizes

Frequently Asked Questions

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