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Rabbit anti-MSK1 (pS212) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MSK1 (pS212)

CAS Number

9007-83-4

Synonyms

MSK1; Ribosomal protein S6 kinase alpha-5; S6K-alpha-5; 90 kDa ribosomal protein S6 kinase 5; Nuclear mitogen- and stress-activated protein kinase 1; RSK-like protein kinase; RSKL

Host

Rabbit

Reactivity

H, M

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MSK1 (pS212) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of MSK1 (pS212) expression in SHSY5Y (A), NIH3T3 (B) whole cell lysates.

IHC description

Immunohistochemical analysis of MSK1 (pS212) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

9252

Gene ID (Mouse)

73086

SwissProt (Human)

O75582

SwissProt (Mouse)

Q8C050

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