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Rabbit anti-MKK4 (pS257) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to MKK4 (pS257)

CAS Number

9007-83-4

Synonyms

JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK

Host

Rabbit

Reactivity

H, M

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MKK4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/100)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of MKK4 (pS257) expression in K562 (A), Hela (B), MCF7 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of MKK4 (pS257) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

6416

Gene ID (Mouse)

26398

SwissProt (Human)

P45985

SwissProt (Mouse)

P47809

Available Sizes

Frequently Asked Questions

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