What is the catalog number for Rabbit anti-Cone Arrestin Antibody?

The catalog number for Rabbit anti-Cone Arrestin Antibody is DL93987A.

What sizes are available for Rabbit anti-Cone Arrestin Antibody?

Rabbit anti-Cone Arrestin Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-Cone Arrestin Antibody come in?

Rabbit anti-Cone Arrestin Antibody is available in 2 different pack sizes.

Is Rabbit anti-Cone Arrestin Antibody in stock?

Rabbit anti-Cone Arrestin Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-Cone Arrestin Antibody

Product Specifications

Background

Rabbit polyclonal antibody to Cone Arrestin

CAS Number

9007-83-4

Synonyms

ARRX; CAR; Arrestin-C; Cone arrestin; C-arrestin; cArr; Retinal cone arrestin-3; X-arrestin

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cone Arrestin. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of Cone Arrestin expression in human lung (A) whole cell lysates.

IHC description

Immunohistochemical analysis of Cone Arrestin staining in human retina formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of Cone Arrestin staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue) .