Rabbit anti-PKR (pT451) Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PKR (pT451)

CAS Number

9007-83-4

Synonyms

PKR; PRKR; Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; eIF-2A protein kinase 2; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated; PKR; Tyrosine-protein kinase EIF2AK2; p68 kinase

Host

Rabbit

Reactivity

H, M

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human PKR (pT451) . The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of PKR (pT451) expression in HeLa PMA-treated (A), Raw264.7 H2O2-treated (B), NIH3T3 LPS-treated (C) whole cell lysates.

IHC description

Immunohistochemical analysis of PKR (pT451) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.