Rabbit anti-POLR1D Antibody
Product Specifications
Background
Rabbit polyclonal antibody to POLR1D
CAS Number
9007-83-4
Synonyms
DNA-directed RNA polymerases I and III subunit RPAC2; RNA polymerases I and III subunit AC2; AC19; DNA-directed RNA polymerase I subunit D; RNA polymerase I 16 kDa subunit; RPA16; RPC16; hRPA19
Host
Rabbit
Reactivity
H
Immunogen
Recombinant full length protein of human POLR1D
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of POLR1D expression in MCF7 (A), A549 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of POLR1D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
51082
SwissProt (Human)
Q9Y2S0
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
TL308981 1 Kit
Synaptophysin (SYP) Human shRNA Plasmid Kit (Locus ID 6855)
Sign In for Pricing
View Details 
MBS6011582-01 0.05 mg
ZNF521, CT (Zinc Finger Protein 521, Early Hematopoietic Zinc Finger Protein, LYST-interacting Protein 3, EHZF, LIP3)
Sign In for Pricing
View Details MBS6011582-02 5x 0.05 mg
ZNF521, CT (Zinc Finger Protein 521, Early Hematopoietic Zinc Finger Protein, LYST-interacting Protein 3, EHZF, LIP3)
Sign In for Pricing
View Details 