Rabbit anti-POLR1D Antibody
Product Specifications
Background
Rabbit polyclonal antibody to POLR1D
CAS Number
9007-83-4
Synonyms
DNA-directed RNA polymerases I and III subunit RPAC2; RNA polymerases I and III subunit AC2; AC19; DNA-directed RNA polymerase I subunit D; RNA polymerase I 16 kDa subunit; RPA16; RPC16; hRPA19
Host
Rabbit
Reactivity
H
Immunogen
Recombinant full length protein of human POLR1D
Clonality
Polyclonal
Applications
WB, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/2000), IF/IC (1/50 - 1/100)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of POLR1D expression in MCF7 (A), A549 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of POLR1D staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
51082
SwissProt (Human)
Q9Y2S0
Available Sizes
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