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Rabbit anti-HARS2 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to HARS2

CAS Number

9007-83-4

Synonyms

HARSL; HARSR; HO3; Probable histidine--tRNA ligase, mitochondrial; Histidine--tRNA ligase-like; Histidyl-tRNA synthetase; HisRS

Host

Rabbit

Reactivity

H

Immunogen

Recombinant full length protein of human HARS2

Clonality

Polyclonal

Applications

WB, IHC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

WB Description

Western blot analysis of HARS2 expression in HepG2 (A), mouse kidney (B), mouse heart (C), rat liver (D), rat brain (E) whole cell lysates.

IHC description

Immunohistochemical analysis of HARS2 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

23438

SwissProt (Human)

P49590

Available Sizes

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