Rabbit anti-DNA Ligase 1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to DNA Ligase 1
CAS Number
9007-83-4
Synonyms
DNA ligase 1; DNA ligase I; Polydeoxyribonucleotide synthase [ATP] 1
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Ligase 1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of DNA Ligase 1 expression in 22RV1 (A), MCF7 (B) whole cell lysates.
IHC description
Immunohistochemical analysis of DNA Ligase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3978
SwissProt (Human)
P18858
Available Sizes
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