Rabbit anti-p53 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to p53
CAS Number
9007-83-4
Synonyms
P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human p53. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of p53 expression in Hela (A), HEK293T (B) whole cell lysates.
IHC description
Immunohistochemical analysis of p53 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7157
SwissProt (Human)
P04637
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