Rabbit anti-CDC25A (pS75) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to CDC25A (pS75)
CAS Number
9007-83-4
Synonyms
M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A
Host
Rabbit
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC25A. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IHC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Store at -20°C. Avoid repeated freeze / thaw cycles.
WB Description
Western blot analysis of CDC25A (pS75) expression in HEK293T UV-treated (A), Jurkat UV-treated (B), NIH3T3 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of CDC25A (pS75) staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
993
SwissProt (Human)
P30304
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