Human IL-34 Protein, His tag (Animal-Free)

Interleukin-34 (IL-34) is a 30 kDa cytokine with 223 amino acid residues. IL-34 is a ligand of CSF-1R and secreted from CSF-1R expressing cells like myeloid cells, epithelial cells, endothelial cells, fibroblasts, and neurons. IL-34 mediates cell survival, proliferation, cytokine/chemokine expression, and migration via activating several signaling pathways. Moreover, it also plays a critical role in developing and maintaining the mononuclear phagocytic system.

Product Specifications

Background

Interleukin-34 (IL-34) is a ligand of CSF-1R and secreted from CSF-1R expressing cells like myeloid cells, epithelial cells, endothelial cells, fibroblasts, and neurons. IL-34 mediates cell survival, proliferation, cytokine/chemokine expression, and migration via activating several signaling pathways. Moreover, it also plays a critical role in developing and maintaining the mononuclear phagocytic system

Product Name Alternative

C16orf77, IL34

Accession Number

Q6ZMJ4

Tag

IL-34

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Measure by its ability to induce human peripheral blood monocytes (PBMCs) proliferation.The ED50 for this effect is < 30 pg/mL

Molecular Weight

26.2 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Human IL-34 consists of 222 amino acids and predicts a molecular mass of 26.2 kDa

Formulation

Lyophilized from PBS, pH 8.0

Applications Notes

Human

Other Product Names

C16orf77, IL34

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously