Mouse anti Human Cytochrome P450 3A5

CYP3A genes encode monooxygenases, enzymes which catalyze drug metabolism and the synthesis of cholesterol, steroids and other lipids. CYP3A (cytochrome P450, family 3, subfamily A), the most abundant p450 enzyme in Human liver, is responsible for the metabolism of more than 50% of all clinical drugs. CYP3A members localize in organs that associate with drug disposition, including the liver, gastrointestinal tract and kidney. The CYP3A cluster consists of four genes: CYP3A43, CYP3A4, CYP3A7 and CYP3A5, and two pseudogenes: CYP3A5P1 and CYP3A5P2. The CYP3A cluster maps to gene locus 7q21.3-q22.1.

Antibody can be used for Western blotting (1-2 µg/mL) and immunohistochemistry on formalin-fixed, paraffin-embedded tissues (1-5 µg/mL). When used in conjunction with CYP3A7 (Cat. No. X2048M), can differentiate between the CYP3A family members, 3A5 and 3A7. Optimal concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Kumarakasingham, M., et al. (2005). Cytochrome p450 profile of colorectal cancer: Identification of markers of prognosis. Clin. Cancer Res. 11(10);3758-3765. 2. McCune, J.S., et al (2005). Contribution of CYP3A5 to hepatic and renal ifosfamide N-dechloroethlation. Drug Metab. Dispos. 33(7);1074-1081. 3. Kim, K.A., et al. (2007). Effect of polymorphic CYP3A5 genotype on the single-dose simvastatin pharmacokinetics in healthy subjects. J. Cin. Pharmacol. 47(1);87-93