Cytokeratin 5+6 Recombinant Rabbit Monoclonal Antibody [PSH0-68]
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.
Product Specifications
CAS Number
9000-83-3
Product Name Alternative
Abbreviation
CK-6A CK-6D K6A CK-6B K6B KRT6B CK-5 K5 KRT5
Swiss Prot
P02538 Human, P04259 Human, P13647 Human
Cellular Locus
Cytoskeleton.
Host
Rabbit
Species Reactivity
Human
Immunogen
Synthetic peptide within human Cytokeratin 6 186-235.
Isotype
IgG
Clone
PSH0-68
Conjugation
Non-conjugated
Type
Recombinant Rabbit monoclonal Antibody
Applications
WB, IF-Cell, FC
Positive Control
A431 cell lysate, NCI-H226 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, HeLa, A431.
Concentration
1 mg/mL
Dilution
WB: 1:1,000 ;IF-Cell: 1:100 ;FC: 1:500-1:1,000
Purity
Protein A affinity purified.
Form
Liquid
Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 60 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
Yes
Frequently Asked Questions
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