DKW Basal Salts With Macronutrients and Micronutrients

With Macronutrients and Micronutrients. As Described by Driver and Kuniyuki (1984) and McGranahan et al. (1987)

Product Specifications

Synonyms

Driver and Kuniyuki Walnut medium

HS Code

3824.99

Applications

Multiple shoot induction Callus-mediated shoot regeneration Improved success rates for specific plant species (e.g., turmeric and walnut) Used to tissue culture woody plants

Assay Protocol

Follow the steps below to prepare 1 liter of tissue culture media using DKW media: Take a beaker and add 800 ml of distilled water to it. To the water add 5.36 grams of PCT’s DKW Media, 30 grams of sugar, and PPM™ (generally 1-2 ml/L). Make up the volume of the media to 1000ml. Adjust the pH of the media between 5.4-5.8. Add gelling agent (agar 6-8 g/L or gellan gum 3-4 g/L) to the media and keep stirring. Autoclave the medium for 20 minutes at 15 psi and 121°C. Your media is ready!

pH

4.8 ± 0.2

Solubility

Water (Appear as colorless to slightly yellow solution, clear, complete)

Molecular Weight

5.36 g/L

Features & Benefits

Easy to use Supply nutrients required by in vitro plants for their proper growth and development Lower nitrogen content and higher sulfate content Multiple shoot induction Callus-mediated shoot regeneration

Storage Conditions

Dehydrated plant tissue culture media is hygroscopic and should be protected from sunlight and moisture. Store the prepared medium at 2-8°C away from direct light. Medium should be used before the expiry date. The entire volume of each bottle should be used immediately after opening, or else the unused portion should be stored in desiccators and refrigerated at 2-8°C.