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Vimentin Rabbit Polyclonal Antibody

Vimentin antibody detects vimentin, a type III intermediate filament protein with a predicted molecular weight of 54 kDa. Vimentin is highly expressed in cells of mesodermal origin as well as in some ectodermal cells, and is often used as a marker of mesenchymal stem cells. It is also utilized in combination with E-cadherin to study the morphological and phenotypic changes in cells undergoing epithelial–mesenchymal transition during development and tumor metastasis. In addition, vimentin antibody is a valuable reagent to stain neuronal precursors and developing radial glia cells.

Product Specifications

Product Name Alternative

Vimentin

UniProt

P08670

Reactivity

Canine, Feline, Human, Mouse, Rat

Immunogen

Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

Target

Vimentin

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified by antigen-affinity chromatography.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Dilution

WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Form

Liquid

Molecular Weight

54

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive control: 293T

Tested Applications

ELISA, ICC, IHC, IHC-Fr, IHC-P, IP, WB

Host or Source

Rabbit

Preservative

PBS, 1% rAlbumin, 20% Glycerol, 0.025% ProClin 300.

Isotype

IgG

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