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PDGFRA / PDGFR Alpha Antibody (aa1000-C-term)

PDGFRA / PDGFR Alpha Antibody (aa1000-C-term)

Product Specifications

Product Name Alternative

PDGFRA, CD140A, CD140a antigen, PDGFalphaR, PDGFR2, PDGFRalpha, AlphaPDGFR, RHEPDGFRA, PDGF receptor alpha, PDGFR-2, PDGFRA/BCR fusion, APDGFR, Pdgf alpha-receptor, PDGF-R-alpha, PDGFR-alpha

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to C-Terminus of human PDGF Receptor alpha.

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Immunoaffinity purified

Dilution

IHC, IHC-P (1:100), IP, WB (1:250 - 1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Further information: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:250-1:1000 dilution detected PDGF Receptor a doublet (150/170kD) in 3T3/NIH cell lysate. 3T3/NIH cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PDGF Receptor a (1:500 dilution) . Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5-10 ul immunoprecipitates PDGF Receptor a from 500 ug of 3T3/NIH RIPA lysate.

Tested Applications

IHC, IHC-P, IP, WB

Host or Source

Rabbit

Preservative

0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide

Isotype

IgG

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