Anti-Hu CD272 PE

CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.

Product Specifications

Certification

RUO

Reactivity

Human, Non-human primates

Immunogen

Cells transfected with human CD272

Target Antigen

CD272

Clone

MIH26

Applications

FC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Ramsay AG , Clear AJ, Fatah R, Gribben JG: Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood. 2012 Aug 16;120 (7) :1412-21., URL: https://pubmed.ncbi.nlm.nih.gov/22547582/?from_term=ramsay+ag+clear+aj&from_pos=4, *Zhang Z, Xu X, Lu J, Zhang S, Gu L, Fu J, Jin L, Li H, Zhao M, Zhang J, Wu H, Su L, Fu YX, Wang FS: B and T lymphocyte attenuator down-regulation by HIV-1 depends on type I interferon and contributes to T-cell hyperactivation. J Infect Dis. 2011 Jun 1;203 (11) :1668-78., URL: https://pubmed.ncbi.nlm.nih.gov/21592997/?from_term=zhang+z+xu+x+gu+l+jin+l&from_pos=2, *Šedý JR, Balmert MO, Ware BC, Smith W, Němčovičová I, Norris PS, Miller BR, Aivazian D, Ware CF: A herpesvirus entry mediator mutein with selective agonist action for the inhibitory receptor B and T lymphocyte attenuator. J Biol Chem. 2017 Dec 22;292 (51) :21060-21070., URL: https://pubmed.ncbi.nlm.nih.gov/29061848/?from_single_result=%C5%A1ed%C3%BD+jr+balmert+mo+ware+bc&expanded_search_query=%C5%A1ed%C3%BD+jr+balmert+mo+ware+bc, *Piancone F , Saresella M, Marventano I , La Rosa F , Zoppis M , Agostini S , Longhi R , Caputo D , Mendozzi L , Rovaris M , Clerici M: B lymphocytes in multiple sclerosis: Bregs and BTLA/CD272 expressing-CD19+ lymphocytes modulate disease severity. Sci Rep. 2016 Jul 14;6:29699. doi: 10.1038/srep29699., URL: https://pubmed.ncbi.nlm.nih.gov/27412504/, *Cairo C, Surendran N, Harris KM, Mazan-Mamczarz K, Sakoda Y, Diaz-Mendez F, Tamada K, Gartenhaus RB, Mann DL, Pauza CD: Vγ2Vδ2 T cell costimulation increases NK cell killing of monocyte-derived dendritic cells. Immunology. 2014 Sep 16;144 (3) :422-430., URL: https://pubmed.ncbi.nlm.nih.gov/25227493/?from_single_result=cairo+c+surendran+n&expanded_search_query=cairo+c+surendran+n

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The mouse monoclonal antibody MIH26 recognizes an extracellular epitope of CD272, a transmembrane glycoprotein serving as a negative regulator of the activation in various leukocyte types.