Anti-BNIP3 Antibody

Rabbit Polyclonal Antibody specific to BNIP3

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-BNIP3 Rabbit Polyclonal Antibody

Gene Name

BNIP3

Gene ID

664

NCBI Gene ID

UniProt

Q12983

Accession Number

NP_004043.3

Cellular Locus

Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Note=Coexpression with the EIB 19-kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to amino acids at the C-terminus of human BNIP3.

Target Antigen

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Target

BNIP3

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. {PubMed:19935772, PubMed:22292033}.

Additionnal Information

Immunoblotting: use at 1ug/mL. A band of ~30kDa is detected; an additional ~60kDa band, complexed BNIP3, may also be detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and mouse BNIP3.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

BNIP3 is a member of the apoptotic Bcl-2 protein family that is involved in both necrosis and apoptosis. Humans and other animals, as well as lower eukaryotes, encode several BNIP3 paralogues including human BNIP3L which induces apoptosis by interacting with viral and cellular anti-apoptosis proteins. BNIP3 interacts with the E1B 19kDa protein, which is responsible for the protection of virally induced cell death, as well as with E1B-like sequences of BCL2 which is also an apoptotic protector. The BNIP3 gene contains a BH3 domain and a transmembrane domain that have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by the BNIP3 gene is known to induce apoptosis, even in the presence of BCL2.