Anti-NR2B Glutamate Receptor Antibody

Mouse Monoclonal Antibody specific to NR2B Glutamate Receptor

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GluN2B, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B

Gene Name

Grin2b

NCBI Gene ID

UniProt

Q00960

Cellular Locus

Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Late endosome, Lysosome, Cytoplasm, cytoskeleton

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein aa 20-271 (extracellular N-terminus) of rat NR2B (accession no. Q00960). This sequence is 99

Target Antigen

Glutamate receptor ionotropic, NMDA 2B

Target

NR2B Glutamate Receptor

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, IHC, ICC/IF, IP, AM

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:1350383, PubMed:19910922, PubMed:21677647, PubMed:24876489, PubMed:27135925, PubMed:27916457). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable). In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity (By similarity). {UniProtKB:Q01097, PubMed:1350383, PubMed:19910922, PubMed:21677647, PubMed:24876489, PubMed:27135925, PubMed:27916457, ECO:0000305}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~166kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat NR2B. It does not cross-react with NR2A.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

Glutamate receptors of the NMDA subtype are involved in several physiological and pathological processes in the brain. Functional NMDA receptors are heteromultimeric complexes of the NR1 subunit and one or more of the four NR2 subunits (NR2A-D). Coexpression of both subunit types are required for the for- mation of fully functional channels that are inserted into the plasma membrane. The NR2 subunit determines characteristics of the channel such as conductance, mean open time, and sensitivity to Mg+2. The ability of NR1 and NR2 subunit combinations to flux Ca+2 is essential to the role that NMDA receptors play in synaptic plasticity and neurotoxicity.