Rabbit anti Human DNA Topoisomerase 1

DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Antibody can be used for immunohistochemistry at 5-10 µg/mL on paraffin-embedded tissues. Optimal concentration should be evaluated by serial dilutions.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Oddou, P., et al. 'Monoclonal antibodies neutralizing mammalian DNA topoisomerase I activity.' Eur. J. Biochem. 1988, 177, 523-529 2. Redinbo, M.R., et al. 'Novel insights into catalytic mechanism from a crystal structure of Human topoisomerase I in complex with DNA.' Biochemistry 2000, 39, 6832-6840 3. Eisenreich, A., et al. 'Cdc2-like kinases and DNA topoisomerase I regulate alternative splicing of tissue factor in Human endothelial cells.' Circ. Res. 2009, 104, 589-599