Citraconic anhydride

Citraconic anhydride (Methylmaleic anhydride) is a derivative of maleic anhydride (HY-Z0060) and novel antigen retrieval solution. Citraconic anhydride reversibly blocks protein amino groups, stabilizing specific enzymes and improving their catalytic performance. Citraconic anhydride reacts with free amino groups on proteins (especially lysine residues), converting positively charged NH3+ into carboxyl groups, thereby disrupting methylene bridge crosslinks caused by Formaldehyde during antigen retrieval. Citraconic anhydride functionalizes Isotactic polypropylene. Citraconic anhydride precisely responds to pH changes to achieve reversible modification. Citraconic anhydride is irritating to skin and eyes[1][2][3][4][5].

Product Specifications

CAS Number

[616-02-4]

Product Name Alternative

Methylmaleic anhydride

UNSPSC

12352200

Hazard Statement

H314, H317, H334

Target

Biochemical Assay Reagents

Type

Biochemical Assay Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/citraconic-anhydride.html

Purity

99.81

Solubility

DMSO : ≥ 100 mg/mL

Smiles

O=C1OC(C(C)=C1)=O

Molecular Formula

C5H4O3

Molecular Weight

112.08

Precautions

H314, H317, H334

References & Citations

[1]Hermanson G T. Chapter 2-functional targets for bioconjugation[J]. Bioconjugate Techniques, 2013, 3: 127-228.|[2]Devrim Y G, et al. Functionalization of isotactic polypropylene with citraconic anhydride. Polymer Bulletin, 2007, 59 (4) : 447-456.|[3]Yankeelov JA Jr, et al. Methylmaleic anhydride as a reversible blocking agent during specific arginine modification. Biochem Biophys Res Commun. 1971 Mar 5;42 (5) :886-91. |[4]Melodia D, et al. Traceless pH-Sensitive Antibody Conjugation Inspired by Citraconic Anhydride. Biomacromolecules. 2022 Dec 12;23 (12) :5322-5329. |[5]Leong AS, et al. Citraconic anhydride: a new antigen retrieval solution. Pathology. 2010 Jan;42 (1) :77-81.