Anti-RAIDD (CT) Antibody

Rabbit Polyclonal Antibody specific to Raidd (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Caspase and RIP adapter with death domain, RIP-associated protein with a death domain

Gene Name

CRADD

Gene ID

8738

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=CRADD">CRADD</a>

UniProt

P78560

Accession Number

NP_001307028.1

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 181-199 at the C-terminus of human RAIDD (accession no. AAB42217).

Target Antigen

Death domain-containing protein CRADD

Target

RAIDD (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, ICC, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis (PubMed:9044836, PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572). Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway (PubMed:8985253). {PubMed:15073321, PubMed:16652156, PubMed:17159900, PubMed:17289572, PubMed:8985253, PubMed:9044836}.

Additionnal Information

Immunoblotting : use at 1ug/mL. Positive control: HeLa cell lysate. Immunohistochemistry: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for ther applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes full-length human RAIDD (22kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

A novel cell death adapter has been identified and designated RAIDD (RIP- associated ICH-1/CED-3-homologous protein with DD) and CRADD (caspase and RIP adapter with DD). RAIDD contains a DD and a CARD (caspase recruitment domain) which interact with RIP and caspase, respectively, to transduce death signals. RAIDD is constitutively expressed in many tissues and mediates apoptosis caused by Fas and TNFR1.