SingleFlowEx CD63 APC

SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.

Product Specifications

Certification

RUO (GMP)

Clone

MEM-259

Applications

FC (QC tested)

Concentration

0.040 mg/mL

Format

APC

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Heneberg P, Riegerová K, Kučera P: Pimecrolimus is a potent inhibitor of allergic reactions to hymenopteran venom extracts and birch pollen allergen in vitro. PLoS One. 2015 Nov 12;10 (11) :e0142953., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=heneberg+p+riegerov%C3%A1+k+ku%C4%8Dera+p , *Cerny J, Feng Y, Yu A, Miyake K, Borgonovo B, Klumperman J, Meldolesi J, McNeil PL, Kirchhausen T: The small chemical vacuolin-1 inhibits Ca (2+) -dependent lysosomal exocytosis but not cell resealing. EMBO Rep. 2004 Sep;5 (9) :883-8. Erratum in: EMBO Rep. 2005 Sep;6 (9) :898. , URL: https://www.ncbi.nlm.nih.gov/pubmed/15332114?dopt=, *Use of this product has not been published., URL:

Other References

*Lin D, Kamsteeg EJ, Zhang Y, Jin Y, Sterling H, Yue P, Roos M, Duffield A, Spencer J, Caplan M, Wang WH: Expression of tetraspan protein CD63 activates protein tyrosine kinase (PTK) and enhances the PTK-induced inhibition of ROMK channels. J Biol Chem. 2008 Jan 22, URL: https://www.ncbi.nlm.nih.gov/pubmed/18211905?ordin, *Kwon MS, Shin SH, Yim SH, Lee KY, Kang HM, Kim TM, Chung YJ: CD63 as a biomarker for predicting the clinical outcomes in adenocarcinoma of lung. Lung Cancer. 2007 Jul;57 (1) :46-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/17350713?ordin, *Israels SJ, McMillan-Ward EM: CD63 modulates spreading and tyrosine phosphorylation of platelets on immobilized fibrinogen. Thromb Haemost. 2005 Feb;93 (2) :311-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15711748?ordin, *Pfistershammer K, Majdic O, Stöckl J, Zlabinger G, Kirchberger S, Steinberger P, Knapp W: CD63 as an activation-linked T cell costimulatory element. J Immunol. 2004 Nov 15;173 (10) :6000-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15528334?ordin, *Mantegazza AR, Barrio MM, Moutel S, Bover L, Weck M, Brossart P, Teillaud JL, Mordoh J: CD63 tetraspanin slows down cell migration and translocates to the endosomal-lysosomal-MIICs route after extracellular stimuli in human immature dendritic cells. Blood. 2004 Aug 15;104 (4) :1183-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/15130945?ordin, *Grützkau A, Smorodchenko A, Lippert U, Kirchhof L, Artuc M, Henz BM: LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells. Cytometry A. 2004 Sep;61 (1) :62-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/15351990?ordin